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脂质体升级版,难转染细胞也能轻松应对,更好用!

名称:新型DNA转染试剂D4000
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货号规格价格
4000-10.75ml1099
4000-21.5ml1999

产品介绍

EntransterTM-D4000是英格恩生物公司(Engreen Biosystem Co,Ltd.)最新研发合成的新型纳米聚合物高效转染试剂,该试剂采用纳米技术合成,是新型的超越脂质体的低毒高效转染试剂。

主要特点

脂质体升级版,难转染细胞也能轻松应对,更好用! 24孔板每孔只需要1ul,价格是脂质体转染试剂的1/3.

用EntransterTM-D4000成功转染的细胞

1、人源,遗传变异细胞和正常组织来源细胞

AsPC-1,BNL CL2,HEK293,CFPEo,HUVEC,HLMEC,H-MVEC,MRC-5,HCS-2/8,HLF,D407,H9C2,SMC

2、人源,肿瘤细胞

A549,HuH-7,HepG2,HME4,A375,Hep-2,SW480,SKOV-3,IGROV1,K562,Jurkat,MCF7,MDA-MB-231,ZR-75-1,TCA8113,Eca-109,SGC-7901,HeLa,SiHa,Ca Ski,CaCo2,SAOS-2,PC-3,SHEP

3、其他动物来源细胞

B16BL6,B16F10,C2C12,C6,CHO,COS-7,CV-1,BHK-21,MDCK,PC12,BAEC,MLE-12,MLE-15,Neuro2A,PGHAM-1,RAW 264.7,EL-4,L929,LLC,3LL,NIH 3T3   

      各细胞系详细转染效率情况

细胞名称来源转染效率(%)
AsPC-1人源,胰脏细胞80%
BNL CL2人源,胚胎肾细胞70%
HEK 293人源,胚胎肾细胞90%
CFPEo人源,气管上皮细胞50%
HUVEC人源,脐带静脉内皮状细胞55%
HLMEC人源,肺血管内皮细胞60%
H-MVEC人源,微血管内皮细胞50%
MRC-5人源,胚胎肺细胞75%
HCS-2/8人源,软骨样细胞40%
HLF人源,肺成纤维细胞65%
D 407人源,视网膜色素上皮细胞60%
A549人源,肺癌细胞90%
HuH-7人源,肝癌细胞63%
HepG2人源,肝癌细胞30%
HME4人源,黑色素瘤细胞90%
A375人源,黑色素瘤细胞70%
Hep-2人源,喉癌细胞70%
SAOS-2人源,骨肉瘤细胞50%
SKOV-3人源,卵巢腺癌细胞60%
IGROV1人源,卵巢癌细胞20%
K562人源,慢性白血病细胞30%
Jurkat人源,T淋巴细胞白血病细胞45%
MCF7人源,乳腺癌细胞55%
MDA-MB-231人源,乳腺癌细胞70%
ZR-75-1人源,乳腺癌细胞70%
TCA8113人源,舌鳞癌细胞90%
Eca-109人源,胃腺癌细胞57%
SGC-7901人源,胃腺癌细胞48%
BGC-823人源,胃腺癌细胞60%
Panc-1人源,胰腺癌细胞40%
HeLa人源,宫颈癌细胞80%
SiHa人源,宫颈癌细胞70%
Ca Ski人源,宫颈表皮样癌细胞80%
CaCo2人源,结肠癌上皮细胞30%
SW480人源,结肠腺癌细胞80%
PC-3人源,前列腺癌细胞30%
SHEP人源,神经母细胞瘤细胞70%
B16BL6小鼠,黑色素瘤细胞90%
B16F10小鼠,黑色素瘤细胞80%
C2C12小鼠,胚胎肌原细胞30%
C6大鼠,脑胶质瘤细胞60%
CHO中国仓鼠,卵巢细胞90%
COS-7猴SV40转化肾细胞80%
CV-1非洲绿猴肾细胞70%
BHK-21仓鼠,肾细胞80%
MDCK犬,肾上皮细胞30%
PC12大鼠,肾上腺嗜铬细胞瘤细胞33%
BAEC牛,动脉内皮细胞50%
MLE-12小鼠,肺上皮细胞70%
MLE-15小鼠,肺上皮细胞70%
Neuro2A小鼠,成神经瘤细胞70%
PGHAM-1仓鼠,胰腺癌细胞90%
RAW   264.7小鼠,巨噬细胞15%
EL-4小鼠,胸腺瘤细胞75%
L929小鼠,成纤维肉瘤细胞60%
LLC小鼠,路易斯肺癌细胞75%
3LL小鼠,路易斯肺癌细胞65%
NIH 3T3小鼠,胚胎纤维原细胞40%
P19小鼠,胚胎肉瘤细胞60%



低细胞毒性

由于EntransterTM-D4000非脂质体,无免疫原性,表现了极低的细胞毒性。

应用本产品文献

1.Cancer Lett, 2016 Jun 28. N-Myc-interacting protein (NMI) negatively regulates epithelial-mesenchymal transition by inhibiting the acetylation of NF-κB/p65.(n-myc-interacting蛋白(NMI)通过抑制NF-κB p65乙酰化负调控上皮间质转化)(IF:5.621 )

2. J Proteome Res. 2015 Aug 7 Proteomic Profiling and Functional Characterization of Multiple Post-Translational Modifications of Tubulin.(微管蛋白蛋白质组学分析及功能研究).

3.FEBS Lett. 2015 Jul 8. Cep70 regulates microtubule stability by interacting with HDAC6.(中心体蛋白Cep70与组蛋白去乙酰化酶HDAC6相互作用调节微管的稳定性)

4. BMC Evol Biol. 2016 Jan 15 Analysis of the erythropoietin of a Tibetan Plateau schizothoracine fish (Gymnocypris dobula) reveals enhanced cytoprotection function in hypoxic environments.(对青藏高原裂腹鱼的促红细胞生成素分析显示缺氧环境下细胞保护作用增强)

5.Chinese Journal of Otology.2009(04):347-351.Study on transfection efficiency and cytotoxicity of 293T cells with different vectors containing Math1-EGFP gene.

6.FEBS Lett. 2010 Jul 2;584(13):2807-15. Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis.

7.Leuk Res. 2011 Feb 3. Specific killing of CCR9 high-expressing acute T lymphocytic leukemia cells by CCL25 fused with PE38 toxin.

8.J Biol Chem. 2011 Jul 27.Cep70 interacts with gamma-tubulin to localize at the centrosome and is critical for mitotic spindle assembly.

9.Cell Biochem Funct. 2011 Oct 26.The effects of myocyte enhancer factor 2A gene on the proliferation, migration and phenotype of vascular smooth muscle cells.

10.Parasit Vectors. 2011 Nov 9;4:213. DNA vaccination with a gene encoding Toxoplasma gondii GRA6 induces partial protection against toxoplasmosis in BALB/c mice.

11.J Cell Sci. 2012 May 28. Regulation of apoptosis by Bat3-enhanced YWK-II protein/APLP2 stability.

12.PLoS One. 2012;7(5):e37452. Exosome-related multi-pass transmembrane protein TSAP6 is a target of rhomboid protease RHBDD1-induced proteolysis.

13.J Appl Toxicol. 2012 Aug;32(8):635-41.Developing in vitro reporter gene assays to assess the hormone receptor activities of chemicals frequently detected in drinking water.

14.Respir Physiol Neurobiol. 2012 Oct 8. Effect of annexin A2 on hepatopulmonary syndrome rat serum-induced proliferation of pulmonary arterial smooth muscle cells.